백서 전상악골 봉합부 확장시 비타민 E에 의한 골형성 촉진

Tancan Uysal; Mihri Amasyali; Huseyin Olmez; Yildirim Karslioglu; Omer Gunhan

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백서 전상악골 봉합부 확장시 비타민 E에 의한 골형성 촉진
Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat

Korean Journal of Orthodontics 2009년 39권 5호 p.337 ~ 347

Tancan Uysal, Mihri Amasyali, Huseyin Olmez, Yildirim Karslioglu, Omer Gunhan,

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( Tancan Uysal ) - Erciyes University Faculty of Dentistry Department of Orthodontics
( Mihri Amasyali ) - Gulhane Military Medical Academy. Ankara, Turkey. Centre of Dental Sciences Department of Orthodontics
( Huseyin Olmez ) - Gulhane Military Medical Academy. Ankara, Turkey. Centre of Dental Sciences Department of Orthodontics
( Yildirim Karslioglu ) - Gulhane Military Medical Academy, Ankara, Turkey. Faculty of Medicine Department of Pathology
( Omer Gunhan ) - Gulhane Military Medical Academy, Ankara, Turkey. Faculty of Medicine Department of Pathology

KMID : 0361920090390050337

Abstract

본 연구는 백서에서 전상악골 봉합부의 확장시 비타민 E (?-토코페롤) 복용의 골형성 효과를 조직계측학적으로 평가하기 위해 시행되었다. 30마리의 50 - 60일된 백서를 6마리씩 5개 집단(대조군과 실험군 4개 집단)으로 분류하였다. 모든 실험 집단에서 50 gm의 힘으로 전상악부를 확장하였다. 장치 장착 1일 후, 대조군(Group I)에는 생리식염수를, 실험군(Group II, Group III, Group IV)에는 ?-토코페롤을 각각 2 mg/kg, 10 mg/kg, 50 mg/kg씩 전상악골 봉합부에 주사하였다. 다른 실험군(Group V)에는 장치 장착 3, 6, 9일 후 각각 10 mg/kg씩의 ?-토코페롤을 주사하였다. 10일간 유지한 뒤 봉합부에서의 골형성을 조직계측학적 방법으로 평가하였다. Kruskal-Wallis rank 및 Mann-Whitney U test를 사용하여 유의수준 0.05에서 통계분석하였다. 신생골 면적, 둘레, 직경의 계측치에서 실험군이 대조군보다 통계적으로 유의성있게 높은 수치를 나타내었다 (p ＜ 0.001). ?-토코페롤을 주사한 집단에서 골 내부구조가 향상되었고, 확장으로 인한 골형성은 주사한 ?-토코페롤 용량에 비례하여 유의성있게 증가되었다. 백서에서 전상악골 봉합부의 확장시 초기 단계에서의 ?-토코페롤 주입은 골형성을 촉진하고 유지기간을 단축시킬 수 있을 것이다.

Objective: The aim of this study was to evaluate the effects of vitamin E (?-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically.

Methods: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of ?-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg ?-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level.

Results: New bone area, bone perimeter, feret’s diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p ＜ 0.001). Bone architecture in ?-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner.

Conclusions: The application of ?-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.